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Methods Enzymol. 2013;530:75-87. doi: 10.1016/B978-0-12-420037-1.00003-8.

Northern blotting.

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Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.


To measure the size and amount of RNA transcribed from a specific gene of interest.


Denaturing agarose gel electrophoresis; Hybridization; Nylon membrane; Radiolabeled RNA probe; Transfer RNA

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