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Braz J Microbiol. 2012 Apr;43(2):581-5. doi: 10.1590/S1517-83822012000200020. Epub 2012 Jun 1.

Diagnosis of paratuberculosis in cattle: microbiological culture, serology and PCR.

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Departamento de Veterinária, Universidade Federal de Viçosa , Viçosa, MG , Brasil.


The aim of this study was to confirm clinical diagnosis of paratuberculosis in two cows showing suggestive clinical signs of the disease. Based on clinical signs, in culture and in IS900 PCR results from the individual milk samples it was possible to diagnose paratuberculosis in the cows studied.


IS900; ISMav2; Mycobacterium avium subspecies paratuberculosis (MAP); cattle; milk

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