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J Clin Microbiol. 2013 Dec;51(12):4193-5. doi: 10.1128/JCM.01876-13. Epub 2013 Sep 11.

Evaluation of pneumococcal serotyping by multiplex PCR and quellung reactions.

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1
Cleveland Clinic, Cleveland, Ohio, USA.

Abstract

Screening of 1,750 pneumococcal isolates for common serotypes by PCR was followed by Quellung reaction analysis of PCR-negative isolates with a comparison to the conventional (Quellung reaction only) approach. PCR agreed with Quellung reaction results for 99% of isolates. The sequential PCR/Quellung reaction algorithm is accurate and more cost-effective than the conventional approach.

PMID:
24025905
PMCID:
PMC3838036
DOI:
10.1128/JCM.01876-13
[Indexed for MEDLINE]
Free PMC Article
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