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Genomics. 2013 Nov-Dec;102(5-6):472-8. doi: 10.1016/j.ygeno.2013.08.007. Epub 2013 Sep 7.

Identification and characterization of microRNAs in the developing maize endosperm.

Author information

1
Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
2
College of Life Science, Sichuan Agricultural Universities, Ya'an, Sichuan 625014, China.
3
College of Agronomy, Sichuan Agricultural University, Chengd u, Sichuan 611130, China.
4
Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address: yubihuang@sohu.com.

Abstract

MicroRNAs (miRNAs) are non-coding RNAs that are approximately 20-22 nucleotides long. miRNAs have been shown to be important regulators that control a large variety of biological functions in eukaryotic cells. To investigate the roles of miRNAs in maize endosperm development, two small RNA libraries of maize endosperm at two developmental stages were sequenced. A total of 17,773,394 and 18,586,523 small RNA raw reads were obtained, respectively. Further analysis identified and characterized 95 known miRNAs belonging to 20 miRNA families. In addition, 18 novel miRNAs were identified and grouped into 11 families. Potential targets for 5 of the novel miRNA families were successfully predicted. We had also identified 12 corresponding miRNAs* of these novel miRNAs. In summary, we investigated expression patterns of miRNA in maize endosperm at key developmental stages and identified miRNAs that are likely to playing an important role in endosperm development.

KEYWORDS:

Endosperm; Identification; Maize; Sequencing; miRNA

PMID:
24021532
DOI:
10.1016/j.ygeno.2013.08.007
[Indexed for MEDLINE]
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