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PLoS One. 2013 Sep 3;8(9):e74763. doi: 10.1371/journal.pone.0074763. eCollection 2013.

A forward genetic approach in Chlamydomonas reinhardtii as a strategy for exploring starch catabolism.

Author information

1
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576, CNRS- Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.

Abstract

A screen was recently developed to study the mobilization of starch in the unicellular green alga Chlamydomonas reinhardtii. This screen relies on starch synthesis accumulation during nitrogen starvation followed by the supply of nitrogen and the switch to darkness. Hence multiple regulatory networks including those of nutrient starvation, cell cycle control and light to dark transitions are likely to impact the recovery of mutant candidates. In this paper we monitor the specificity of this mutant screen by characterizing the nature of the genes disrupted in the selected mutants. We show that one third of the mutants consisted of strains mutated in genes previously reported to be of paramount importance in starch catabolism such as those encoding β-amylases, the maltose export protein, and branching enzyme I. The other mutants were defective for previously uncharacterized functions some of which are likely to define novel proteins affecting starch mobilization in green algae.

PMID:
24019981
PMCID:
PMC3760859
DOI:
10.1371/journal.pone.0074763
[Indexed for MEDLINE]
Free PMC Article

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