Format

Send to

Choose Destination
Chemistry. 2013 Sep 27;19(40):13288-93. doi: 10.1002/chem.201302429. Epub 2013 Sep 9.

Real-time DNP NMR observations of acetic acid uptake, intracellular acidification, and of consequences for glycolysis and alcoholic fermentation in yeast.

Author information

1
Albeda Research, Gamle Carlsberg Vej 10, 1799 Copenhagen V (Denmark).

Abstract

Uptake and upshot in vivo: Straightforward methods that permit the real-time observation of organic acid influx, intracellular acidification, and concomitant effects on cellular-reaction networks are crucial for improved bioprocess monitoring and control. Herein, dynamic nuclear polarization (DNP) NMR is used to observe acetate influx, ensuing intracellular acidification and the metabolic consequences on alcoholic fermentation and glycolysis in living cells.

KEYWORDS:

NMR spectroscopy; acetate; bioethanol; in vivo spectroscopy; yeast fermentation

PMID:
24019026
DOI:
10.1002/chem.201302429
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center