Format

Send to

Choose Destination
Cancer Lett. 2014 Feb 1;343(1):6-13. doi: 10.1016/j.canlet.2013.09.002. Epub 2013 Sep 7.

Knockdown of CRM1 inhibits the nuclear export of p27(Kip1) phosphorylated at serine 10 and plays a role in the pathogenesis of epithelial ovarian cancer.

Author information

1
Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, PR China; Department of Obstetrics and Gynecology, Wuxi Maternal and Child Health Hospital, Nanjing Medical University, Wuxi 214000, PR China.
2
Department of Obstetrics and Gynecology, Nantong University Cancer Hospital, Nantong University, Nantong 226001, Jiangsu, PR China.
3
Department of Obstetrics and Gynecology, Wuxi Maternal and Child Health Hospital, Nanjing Medical University, Wuxi 214000, PR China.
4
Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, PR China.
5
Department of Obstetrics and Gynecology, Nantong University Cancer Hospital, Nantong University, Nantong 226001, Jiangsu, PR China. Electronic address: liurong0513@sina.cn.
6
Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, PR China. Electronic address: diwen163@163.com.

Abstract

In a previous study, the nuclear export protein chromosomal region maintenance (CRM1) was correlated with p27(Kip1) in glioma. The aims of the present study were to investigate the expression of CRM1 and pSer10p27 and their functional roles in epithelial ovarian cancer (EOC) tissues. Using immunohistochemical analysis, CRM1 and pSer10p27 expression levels were shown to be associated with histologic stage and grade (P<0.05). High CRM1 and pSer10p27 expression levels were prognostic indicators of overall survival (P<0.05). Knockdown of CRM1 and pSer10p27 expression arrested cell cycle progression and inhibited the proliferation of SKOV3 cells both in vitro and in vivo. These data support the idea that pSer10p27 and CRM1 play cooperative roles in EOC.

KEYWORDS:

Chromosomal region maintenance; Epithelial ovarian cancer; Phosphorylation; Proliferation; p27(Kip1)

PMID:
24018641
DOI:
10.1016/j.canlet.2013.09.002
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center