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Methods Mol Biol. 2013;1077:203-15. doi: 10.1007/978-1-62703-637-5_14.

Accurate measurement of nicotinamide adenine dinucleotide (NAD⁺) with high-performance liquid chromatography.

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1
Center for Human Nutrition, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.

Abstract

Nicotinamide adenine dinucleotide (NAD(+)) plays a critical role in regulating numerous biological and physiological pathways including metabolism, inflammation, cancer, and aging in mammals. Here we describe a highly quantitative method with reverse-phase high-performance liquid chromatography (HPLC) for the determination of NAD(+) levels in cells and tissues. This methodology provides accurate, reliable, and reproducible results of NAD(+) measurement, which enables us to analyze various pathophysiological changes in NAD(+) levels in vitro and in vivo.

PMID:
24014409
PMCID:
PMC3935825
DOI:
10.1007/978-1-62703-637-5_14
[Indexed for MEDLINE]
Free PMC Article
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