Format

Send to

Choose Destination
Methods Mol Biol. 2013;1077:121-31. doi: 10.1007/978-1-62703-637-5_8.

Targeted quantitation of acetylated lysine peptides by selected reaction monitoring mass spectrometry.

Author information

1
Buck Institute for Research on Aging, Novato, CA, USA.

Abstract

Mass spectrometry (MS) allows for the large-scale identification of multiple peptide analytes in complex mixtures. However, the low abundance of acetylated peptides in the overall mixture requires an enrichment step. After enrichment, the resulting acetylated peptides of interest can be quantitated using selected reaction monitoring (SRM)-MS with stable isotope dilution. Here, we describe the enrichment of lysine acetylated peptides from typsin digested mouse liver mitochondria, and the targeted quantitation of a known lysine acetylation site in succinate dehydrogenase A using SRM-MS on a triple quadrupole instrument.

PMID:
24014403
PMCID:
PMC4378680
DOI:
10.1007/978-1-62703-637-5_8
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center