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Methods Mol Biol. 2013;1068:211-20. doi: 10.1007/978-1-62703-619-1_15.

Calcium imaging of vomeronasal organ response using slice preparations from transgenic mice expressing G-CaMP2.

Author information

1
Stowers Institute for Medical Research, Kansas City, MO, USA.

Abstract

The vomeronasal organ (VNO) in vertebrate animals detects pheromones and interspecies chemical signals. We describe in this chapter a Ca(2+) imaging approach using transgenic mice that express the genetically encoded Ca(2+) sensor G-CaMP2 in VNO tissue. This approach allows us to analyze the complex patterns of the vomeronasal neuron response to large number of chemosensory stimuli.

PMID:
24014364
PMCID:
PMC4492248
DOI:
10.1007/978-1-62703-619-1_15
[Indexed for MEDLINE]
Free PMC Article

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