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Chem Commun (Camb). 2013 Oct 21;49(82):9458-60. doi: 10.1039/c3cc43985b.

Fluorescence turn-on detection of a protein using cytochrome c as a quencher.

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1
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.

Abstract

A new approach for the sensitive and selective sensing of a protein has been developed. Cytochrome c could quench the fluorescence of a fluorophore labeled aptamer efficiently. The specific binding between the aptamer and the target protein resulted in a turn-on emission signal, which could be used for protein quantification.

PMID:
24013356
DOI:
10.1039/c3cc43985b
[Indexed for MEDLINE]
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