Format

Send to

Choose Destination
Biotechnol Appl Biochem. 2013 Sep-Oct;60(5):527-35. doi: 10.1002/bab.1121. Epub 2013 Sep 6.

Polymer-based alternative method to extract bromelain from pineapple peel waste.

Author information

1
Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Science, University of São Paulo, São Paulo, Brazil.

Abstract

Bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (Ananas comosus Merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. The use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. We aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two-phase system formed by polyethylene glycol (PEG) and poly(acrylic acid). In this work, bromelain partitioned preferentially to the top/PEG-rich phase and, in the best condition, achieved a yield of 335.27% with a purification factor of 25.78. The statistical analysis showed that all variables analyzed were significant to the process.

KEYWORDS:

aqueous two-phase system; bromelain; pineapple waste; poly(acrylic acid); polyethylene glycol

PMID:
24011234
DOI:
10.1002/bab.1121
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center