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Pigment Cell Melanoma Res. 2014 Jan;27(1):103-12. doi: 10.1111/pcmr.12164. Epub 2013 Oct 9.

A melanoma immune response signature including Human Leukocyte Antigen-E.

Author information

1
Laboratory of Immunology, Regina Elena National Cancer Institute, Rome, Italy.

Abstract

Paired cultures of early-passage melanoma cells and melanocytes were established from metastatic lesions and the uninvolved skin of five patients. In this stringent autologous setting, cDNA profiling was used to analyze a subset of 1477 genes selected by the Gene Ontology term 'immune response'. Human Leukocyte Antigen E (HLA-E) was ranked 19th among melanoma-overexpressed genes and was embedded in a transformation signature including its preferred peptide ligand donors HLA-A, HLA-B, HLA-C, and HLA-G. Mostly undetectable in normal skin and 39 nevi (including rare and atypical lesions), HLA-E was detected by immunohistochemistry in 17/30 (57%) and 32/48 (67%) primary and metastatic lesions, respectively. Accordingly, surface HLA-E was higher on melanoma cells than on melanocytes and protected the former (6/6 cell lines) from lysis by natural killer (NK) cells, functionally counteracting co-expressed triggering ligands. Although lacking HLA-E, melanocytes (4/4 cultures) were nevertheless (and surprisingly) fully protected from NK cell lysis.

KEYWORDS:

HLA-E; NK cells; cDNA arrays; melanocytes; melanoma

PMID:
24011128
DOI:
10.1111/pcmr.12164
[Indexed for MEDLINE]

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