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Biomed Opt Express. 2013 Jul 9;4(8):1285-93. doi: 10.1364/BOE.4.001285. eCollection 2013.

In vivo two-photon imaging of the mouse retina.

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Center for Visual Science, University of Rochester, Rochester, New York 14627, USA ; The Institute of Optics, University of Rochester, Rochester, New York 14620, USA.


Though in vivo two-photon imaging has been demonstrated in non-human primates, improvements in the signal-to-noise ratio (SNR) would greatly improve its scientific utility. In this study, extrinsic fluorophores, expressed in otherwise transparent retinal ganglion cells, were imaged in the living mouse eye using a two-photon fluorescence adaptive optics scanning laser ophthalmoscope. We recorded two orders of magnitude greater signal levels from extrinsically labeled cells relative to previous work done in two-photon autofluorescence imaging of primates. Features as small as single dendrites in various layers of the retina could be resolved and predictions are made about the feasibility of measuring functional response from cells. In the future, two-photon imaging in the intact eye may allow us to monitor the function of retinal cell classes with infrared light that minimally excites the visual response.


(170.0110) Imaging systems; (180.4315) Nonlinear microscopy; (330.4460) Ophthalmic optics and devices

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