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J Extracell Vesicles. 2013 Jan 10;2. doi: 10.3402/jev.v2i0.19861. eCollection 2013.

How pure are your vesicles?

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Institute of Cancer and Genetics, School of Medicine, Velindre Cancer Centre, Cardiff University, Cardiff, UK.


We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step. Also, the approach may aid in comparing/standardising vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers. We herein propose some criteria to aid in the definition of pure vesicles.


extracellular vesicles; nano-particle tracking; sample purity; vesicle counting

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