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J Extracell Vesicles. 2013 Jan 10;2. doi: 10.3402/jev.v2i0.19861. eCollection 2013.

How pure are your vesicles?

Author information

1
Institute of Cancer and Genetics, School of Medicine, Velindre Cancer Centre, Cardiff University, Cardiff, UK.

Abstract

We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step. Also, the approach may aid in comparing/standardising vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers. We herein propose some criteria to aid in the definition of pure vesicles.

KEYWORDS:

extracellular vesicles; nano-particle tracking; sample purity; vesicle counting

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