Format

Send to

Choose Destination
Chirality. 2013 Dec;25(12):917-22. doi: 10.1002/chir.22234. Epub 2013 Sep 6.

Enantioselective bioaccumulation of diniconazole in Tenebrio molitor larvae.

Author information

1
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China; College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, China.

Abstract

The enantioselective bioaccumulation of diniconazole in Tenebrio molitor Linne larva was investigated with liquid chromatography tandem mass spectrometry based on the ChiralcelOD-3R[cellulose tri-(3,5-dimethylphenyl carbamate)] column. In this study we documented the effects of dietary supplementation with wheat bran contaminated by racemic diniconazole at two dose levels of 20 mg kg(-1) and 2 mg kg(-1) (dry weight) in Tenebrio molitor. The results showed that both doses of diniconazole were taken up by Tenebrio molitor rapidly in the first few days, the concentrations of R-enantiomer and S-enantiomer at high doses reached the highest level of 0.55 mg kg(-1) and 0.48 mg kg(-1) , respectively, on the 1(st) d, and the concentrations of them obtained a maxima of 0.129 mg kg(-1) and 0.128 mg kg(-1) at low dose, respectively, on the 3(rd) d, which means that the concentration of diniconazole was proportional to the time of achieving the highest accumulated level. It afterwards attained equilibrium after a sharp decline at both 20 mg kg(-1) and 2 mg kg(-1) of diniconazole. The determination results from the feces of Tenebrio molitor demonstrated that the extraction recovery (ER) values of the high dose group were higher than that of the low dose group and the values were all above 1; therefore, it could be inferred that enantiomerization existed in Tenebrio molitor. Additionally, the biota accumulation factor was used to evaluate the bioaccumulation of diniconazole enantiomers, showing that the bioaccumulation of diniconazole in Tenebrio molitor was enantioselective with preferential accumulation of S-enantiomer.

KEYWORDS:

Tenebrio molitor; bioaccumulation; diniconazole; enantioselectivity

PMID:
24009189
DOI:
10.1002/chir.22234
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center