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J Antimicrob Chemother. 2014 Jan;69(1):85-90. doi: 10.1093/jac/dkt345. Epub 2013 Sep 4.

Evaluation of the AID ESBL line probe assay for rapid detection of extended-spectrum β-lactamase (ESBL) and KPC carbapenemase genes in Enterobacteriaceae.

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1
Institut für Medizinische Mikrobiologie, Universität Zürich, 8006 Zürich, Switzerland.

Abstract

OBJECTIVES:

This study aimed at evaluating the AID ESBL line probe assay for the detection of extended-spectrum β-lactamase (ESBL) and KPC carbapenemase genes in Enterobacteriaceae.

METHODS:

The AID ESBL line probe assay was verified for accuracy of its probes using PCR products from clinical ESBL Enterobacteriaceae strains harbouring TEM, SHV and CTX-M ESBL genes and KPC genes and mutant fusion PCR products generated from Enterobacteriaceae strains containing wild-type (wt) TEM and wt SHV. Sensitivity and specificity was determined testing a set of 424 clinical Enterobacteriaceae strains (including 170 strains negative for TEM, SHV, CTX-M and KPC to evaluate the possibility of false positive signals).

RESULTS:

The line probe assay was shown to detect with 100% accuracy ESBL genes for which oligonucleotide probes are present in the assay. Testing a set of 424 clinical Enterobacteriaceae strains showed 100% sensitivity and specificity for the detection and differentiation of TEM, SHV and CTX-M ESBL genes present in that group. In addition, the line probe assay detected KPC genes accurately.

CONCLUSIONS:

The AID ESBL line probe assay is an accurate and easy-to-use test for the detection of ESBL and KPC genes, which can readily be implemented in the diagnostic laboratory.

KEYWORDS:

CTX-M; KPC; SHV; TEM; antibiotic resistance

PMID:
24004861
DOI:
10.1093/jac/dkt345
[Indexed for MEDLINE]
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