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Biotechniques. 2013 Sep;55(3):137-40. doi: 10.2144/000114078.

Low titer lentiviral transgenesis in rodents with simian immundeficiency virus vector.

Author information

1
Institute of Animal Biotechnology, Agricultural Biotechnology Center, Gödöllő, Hungary.

Abstract

Efficient production of transgenic animals using low-titer lentiviral constructs remains challenging. Here we demonstrate that microinjection of simian immundeficiency virus-derived lentiviral constructs can produce transgenic mice and rats with high efficiency even when using low-titer virus preparations.

KEYWORDS:

Lentiviral transgenesis; SIV; low titer; transgenic mice; transgenic rat

PMID:
24003946
DOI:
10.2144/000114078
[Indexed for MEDLINE]
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