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Neuroscience. 2013 Dec 3;253:221-34. doi: 10.1016/j.neuroscience.2013.08.038. Epub 2013 Aug 31.

Prior cold water swim stress alters immobility in the forced swim test and associated activation of serotonergic neurons in the rat dorsal raphe nucleus.

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1
Department of Psychology, University of New Hampshire, Durham, NH 03824-3567, USA.

Abstract

Prior adverse experience alters behavioral responses to subsequent stressors. For example, exposure to a brief swim increases immobility in a subsequent swim test 24h later. In order to determine if qualitative differences (e.g. 19°C versus 25°C) in an initial stressor (15-min swim) impact behavioral, physiological, and associated neural responses in a 5-min, 25°C swim test 24h later, rats were surgically implanted with biotelemetry devices 1 week prior to experimentation then randomly assigned to one of six conditions (Day 1 (15 min)/Day 2 (5 min)): (1) home cage (HC)/HC, (2) HC/25°C swim, (3) 19°C swim/HC, (4) 19°C swim/25°C swim, (5) 25°C swim/HC, (6) 25°C swim/25°C swim. Core body temperature (Tb) was measured on Days 1 and 2 using biotelemetry; behavior was measured on Day 2. Rats were transcardially perfused with fixative 2h following the onset of the swim on Day 2 for analysis of c-Fos expression in midbrain serotonergic neurons. Cold water (19°C) swim on Day 1 reduced Tb, compared to both 25°C swim and HC groups on Day 1, and, relative to rats exposed to HC conditions on Day 1, reduced the hypothermic response to the 25°C swim on Day 2. The 19°C swim on Day 1, relative to HC exposure on Day 1, increased immobility during the 5-min swim on Day 2. Also, 19°C swim, relative to HC conditions, on Day 1 reduced swim (25°C)-induced increases in c-Fos expression in serotonergic neurons within the dorsal and interfascicular parts of the dorsal raphe nucleus. These results suggest that exposure to a 5-min 19°C cold water swim, but not exposure to a 5-min 25°C swim alters physiological, behavioral and serotonergic responses to a subsequent stressor.

KEYWORDS:

5-HT; 5-hydroxytryptamine, serotonin; ANOVA; DR; DRD; DRI; DRVL/VLPAG; FST; H(2)O(2); HC; LSD; NaN(3); PBS; PBS containing 0.1% Triton X-100; PBST; SS; T(b); Tph; analysis of variance; core body temperature; core body temperature (°C); dH(2)O; depression; distilled H(2)O; dorsal raphe nucleus; dorsal raphe nucleus, dorsal part; dorsal raphe nucleus, interfascicular part; dorsal raphe nucleus, ventrolateral part/ventrolateral periaqueductal gray; forced swim test; home cage; hydrogen peroxide; immunoreactive; ir; least significant difference; phosphate-buffered saline; raphe; serotonin; sodium azide; swim stress; tryptophan hydroxylase

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