Simultaneous isolation of seven compounds from Glehnia littoralis roots by off-line overpressured layer chromatography guided by a TLC antioxidant autographic assay

J Sep Sci. 2013 Nov;36(21-22):3644-50. doi: 10.1002/jssc.201300523. Epub 2013 Sep 24.

Abstract

A combinative method using overpressured layer chromatography (OPLC) and TLC bioautography against O2(•-) was developed to separate antioxidants from Glehnia littoralis roots. Through target-directed isolation by the TLC bioautographic method, seven compounds including five antioxidants were rapidly isolated by OPLC and identified as 1-linoloyl-3-palmitoylglycerol, facarindiol, panaxynol, isoimperatorin, β-sitosterol, scopoletin, and umbelliferone from G. littoralis roots. This OPLC method is a very suitable separation technique for light-sensitive polyacetylenes (panaxynol and facarindiol) with higher isolated yields compared to conventional open column chromatography. This is the first report on the separation of polyacetylenes by OPLC.

Keywords: Antioxidants; Bioautography; Glehnia littoralis; Overpressured layer chromatography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / chemistry
  • Antioxidants / isolation & purification*
  • Apiaceae / chemistry*
  • Autoradiography
  • Chromatography, Thin Layer
  • Plant Roots / chemistry*
  • Polyynes / chemistry
  • Polyynes / isolation & purification*
  • Pressure

Substances

  • Antioxidants
  • Polyynes