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Methods Mol Biol. 2013;1064:17-27. doi: 10.1007/978-1-62703-601-6_2.

Analysis of protein-protein interactions using LUMIER assays.

Author information

1
Genomics and Proteomics Core Facility, German Cancer Research Center, Heidelberg, Germany.

Abstract

Co-affinity purification methods can test whether two proteins physically engage in a complex. The assay principle is to enrich cellular extracts for a first protein by a purification step, and then test if a second protein is enriched as well. This principle has been optimized for use at high-throughput in LUMIER (luminescence-based mammalian interactome mapping) assays, which use luciferase-tags for the detection of the second proteins. This protocol is ideal for miniaturization of the assay on microtiter plates and supports a throughput of several hundred interaction tests per week.

PMID:
23996247
DOI:
10.1007/978-1-62703-601-6_2
[Indexed for MEDLINE]

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