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Methods Mol Biol. 2013;1033:381-400. doi: 10.1007/978-1-62703-487-6_24.

Optimizing E. coli-based membrane protein production using Lemo21(DE3) and GFP-fusions.

Author information

1
Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.

Abstract

Optimizing the conditions for the overexpression of membrane proteins in E. coli and their subsequent purification is usually a laborious and time-consuming process. Combining the Lemo21(DE3) strain, which conveniently allows to identify the optimal expression intensity of a membrane protein using only one strain, and membrane proteins C-terminally fused to Green Fluorescent Protein (GFP) greatly facilitates the production of high-quality membrane protein material for functional and structural studies.

PMID:
23996190
DOI:
10.1007/978-1-62703-487-6_24
[Indexed for MEDLINE]

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