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Bioinformatics. 2013 Nov 15;29(22):2936-7. doi: 10.1093/bioinformatics/btt515. Epub 2013 Aug 31.

Consed: a graphical editor for next-generation sequencing.

Author information

1
Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA.

Abstract

SUMMARY:

The rapid growth of DNA sequencing throughput in recent years implies that graphical interfaces for viewing and correcting errors must now handle large numbers of reads, efficiently pinpoint regions of interest and automate as many tasks as possible. We have adapted consed to reflect this. To allow full-feature editing of large datasets while keeping memory requirements low, we developed a viewer, bamScape, that reads billion-read BAM files, identifies and displays problem areas for user review and launches the consed graphical editor on user-selected regions, allowing, in addition to longstanding consed capabilities such as assembly editing, a variety of new features including direct editing of the reference sequence, variant and error detection, display of annotation tracks and the ability to simultaneously process a group of reads. Many batch processing capabilities have been added.

AVAILABILITY:

The consed package is free to academic, government and non-profit users, and licensed to others for a fee by the University of Washington. The current version (26.0) is available for linux, macosx and solaris systems or as C++ source code. It includes a user's manual (with exercises) and example datasets. http://www.phrap.org/consed/consed.html

CONTACT:

dgordon@uw.edu .

PMID:
23995391
PMCID:
PMC3810858
DOI:
10.1093/bioinformatics/btt515
[Indexed for MEDLINE]
Free PMC Article

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