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Nat Methods. 2013 Oct;10(10):1003-5. doi: 10.1038/nmeth.2633. Epub 2013 Sep 1.

Absolute quantification by droplet digital PCR versus analog real-time PCR.

Author information

1
1] Digital Biology Center, Bio-Rad Laboratories, Inc., Pleasanton, California, USA. [2] [3].

Abstract

Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision (coefficients of variation decreased 37-86%) and improved day-to-day reproducibility (by a factor of seven) of ddPCR but with comparable sensitivity. When we applied ddPCR to serum microRNA biomarker analysis, this translated to superior diagnostic performance for identifying individuals with cancer.

PMID:
23995387
PMCID:
PMC4118677
DOI:
10.1038/nmeth.2633
[Indexed for MEDLINE]
Free PMC Article

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