Format

Send to

Choose Destination
J Photochem Photobiol B. 2013 Nov 5;128:20-6. doi: 10.1016/j.jphotobiol.2013.08.005. Epub 2013 Aug 15.

Study on the interaction of the drug mesalamine with calf thymus DNA using molecular docking and spectroscopic techniques.

Author information

1
Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran. Electronic address: nahidshahabadi@yahoo.com.

Abstract

The interaction of CT-DNA with the drug mesalamine (5-ASA) at physiological pH has been investigated by absorption, emission, circular dichroism (CD), cyclic voltammetry (CV), viscosity studies and molecular modeling. Thermodynamic parameters (ΔH>0 and ΔS<0) indicated that hydrogen bond and van der Waals play main roles in the binding of 5-ASA to CT-DNA. Ethidium bromide (EB) displacement studies revealed that 5-ASA did not have any effect on ethidium bromide (EB) bound DNA which is indicative of groove binding. The results obtained from experimental and molecular modeling showed that 5-ASA is a minor groove binder of DNA and preferentially binds to GC rich regions.

KEYWORDS:

CT-DNA; Groove binding; Mesalamine; Molecular docking

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center