Format

Send to

Choose Destination
See comment in PubMed Commons below
PLoS One. 2013 Aug 26;8(8):e69257. doi: 10.1371/journal.pone.0069257. eCollection 2013.

Tracking single cells in live animals using a photoconvertible near-infrared cell membrane label.

Author information

1
Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States of America.

Erratum in

  • PLoS One. 2013;8(11). doi:10.1371/annotation/c05446df-ee55-4072-940c-543adff42086. Spencer, Joel A [added].

Abstract

We describe a novel photoconversion technique to track individual cells in vivo using a commercial lipophilic membrane dye, DiR. We show that DiR exhibits a permanent fluorescence emission shift (photoconversion) after light exposure and does not reacquire the original color over time. Ratiometric imaging can be used to distinguish photoconverted from non-converted cells with high sensitivity. Combining the use of this photoconvertible dye with intravital microscopy, we tracked the division of individual hematopoietic stem/progenitor cells within the calvarium bone marrow of live mice. We also studied the peripheral differentiation of individual T cells by tracking the gain or loss of FoxP3-GFP expression, a marker of the immune suppressive function of CD4(+) T cells. With the near-infrared photoconvertible membrane dye, the entire visible spectral range is available for simultaneous use with other fluorescent proteins to monitor gene expression or to trace cell lineage commitment in vivo with high spatial and temporal resolution.

PMID:
23990881
PMCID:
PMC3753322
DOI:
10.1371/journal.pone.0069257
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Support Center