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Bioresour Technol. 2013 Nov;147:52-8. doi: 10.1016/j.biortech.2013.07.143. Epub 2013 Aug 6.

H2S removal and bacterial structure along a full-scale biofilter bed packed with polyurethane foam in a landfill site.

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Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address:


Hydrogen sulfide accumulated under a cover film in a landfill site was treated for 7 months by a full-scale biofilter packed with polyurethane foam cubes. Sampling ports were set along the biofilter bed to investigate H2S removal and microbial characteristics in the biofilter. The H2S was removed effectively by the biofilter, and over 90% removal efficiency was achieved in steady state. Average elimination capacity of H2S was 2.21 g m(-3) h(-1) in lower part (LPB) and 0.41 g m(-3) h(-1) in upper part (UPB) of the biofilter. Most H2S was eliminated in LPB. H2S concentration varied along the polyurethane foam packed bed, the structure of the bacterial communities showed spatial variation in the biofilter, and H2S removal as well as products distribution changed accordingly. The introduction of odorants into the biofilter shifted the distribution of the existing microbial populations toward a specific culture that could metabolize the target odors.


Biofiltration; DNA sequence analysis; Hydrogen sulfide; Landfill odors control; Microbial community

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