Enzyme assisted extraction of polysaccharides from the fruit of Cornus officinalis

Carbohydr Polym. 2013 Oct 15;98(1):607-10. doi: 10.1016/j.carbpol.2013.06.036. Epub 2013 Jun 28.

Abstract

Process of enzyme assisted extraction (EAE) of polysaccharides from Cornus officinalis was optimized by response surface methodology (RSM). The influence of four different factors on the yield of C. officinalis polysaccharides (COP) was studied. Results showed that the optimal conditions were compound enzyme amount of 2.15%, extraction pH of 4.2, extraction temperature of 55 °C and extraction time of 97 min. Under these conditions, the COP yield was 9.29±0.31%, which was well in agreement with the value predicted by the model. The three methods, EAE, hot water extraction (HWE), ultrasound-assisted extraction (UAE) for extracting COP by RSM were further compared. Results showed that EAE had the largest yield of polysaccharides with lower equipment cost.

Keywords: Cornus officinalis; Enzyme assisted extraction; Response surface methodology; The yield of Cornus officinalis polysaccharides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chemical Fractionation / methods*
  • Chemical Phenomena
  • Cornus / chemistry*
  • Fruit / chemistry*
  • Hydrolases / metabolism*
  • Polysaccharides / chemistry
  • Polysaccharides / isolation & purification*
  • Ultrasonics
  • Water / chemistry

Substances

  • Polysaccharides
  • Water
  • Hydrolases