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Pest Manag Sci. 2014 Jun;70(6):977-81. doi: 10.1002/ps.3638. Epub 2013 Sep 30.

Identification of an alternative knockdown resistance (kdr)-like mutation, M918L, and a novel mutation, V1010A, in the Thrips tabaci voltage-gated sodium channel gene.

Author information

1
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian, P.R. China; Department of Entomology, Washington State University, Pullman, WA, USA.

Abstract

BACKGROUND:

Knockdown resistance (kdr) has been identified as a main mechanism against pyrethroid insecticides in many arthropod pests including in the onion thrips, Thrips tabaci. To characterize and identify pyrethroid-resistance in onion thrips in Washington state, we conducted insecticide bioassays and sequenced a region of the voltage gated sodium channel gene from several different T. tabaci populations.

RESULTS:

Field collected Thrips tabaci were found to have large variations in resistance to the pyrethroid insecticide lambda-cyhalothrin. We identified two single nucleotide substitutions in our analysis of a partial sequence of the T. tabaci voltage-gated sodium channel gene. One mutation resulted in the non-synonymous substitution of methionine with leucine (M918L), which is well known to be responsible for super knockdown resistance in some pest species. Another non-synonymous substitution, a valine (GTT) to alanine (GCT) replacement at amino acid 1010 (V1010A) was identified in our study and was associated with lambda-cyhalothrin resistance.

CONCLUSION:

We have characterized a known kdr mutation and identified a novel mutation in the voltage-gated sodium channel gene of Thrips tabaci associated with resistance to lambda-cyhalothrin. This gene region and these mutations are expected to be useful in the development of a diagnostic test to detect kdr resistance in many onion thrips populations.

KEYWORDS:

Thrips tabaci; kdr; point mutation; pyrethroid resistance; sodium channel

PMID:
23983138
DOI:
10.1002/ps.3638
[Indexed for MEDLINE]

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