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Biotechnol Bioeng. 2014 Jan;111(1):1-15. doi: 10.1002/bit.25096. Epub 2013 Sep 13.

Expanding the scope of site-specific recombinases for genetic and metabolic engineering.

Author information

1
The Skaggs Institute for Chemical Biology and the Departments of Chemistry and Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California, 92037.

Abstract

Site-specific recombinases are tremendously valuable tools for basic research and genetic engineering. By promoting high-fidelity DNA modifications, site-specific recombination systems have empowered researchers with unprecedented control over diverse biological functions, enabling countless insights into cellular structure and function. The rigid target specificities of many sites-specific recombinases, however, have limited their adoption in fields that require highly flexible recognition abilities. As a result, intense effort has been directed toward altering the properties of site-specific recombination systems by protein engineering. Here, we review key developments in the rational design and directed molecular evolution of site-specific recombinases, highlighting the numerous applications of these enzymes across diverse fields of study.

KEYWORDS:

genome engineering; protein engineering; recombinase

PMID:
23982993
PMCID:
PMC4097888
DOI:
10.1002/bit.25096
[Indexed for MEDLINE]
Free PMC Article

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