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Genetics. 2013 Nov;195(3):1187-91. doi: 10.1534/genetics.113.156299. Epub 2013 Aug 26.

CRISPR/Cas9-targeted mutagenesis in Caenorhabditis elegans.

Author information

1
Department of Biology, Utrecht University, 3584 CH, Utrecht, The Netherlands.

Abstract

The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome.

KEYWORDS:

CRISPR; Caenorhabditis elegans; Cas9; double-strand break; genome engineering

PMID:
23979586
PMCID:
PMC3813849
DOI:
10.1534/genetics.113.156299
[Indexed for MEDLINE]
Free PMC Article
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