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Genetics. 2013 Nov;195(3):1181-5. doi: 10.1534/genetics.113.156075. Epub 2013 Aug 26.

Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system.

Author information

1
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.

Abstract

We adapted the CRISPR-Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the germline of C. elegans.

KEYWORDS:

CRISPR–Cas9; Caenorhabditis elegans; genome editing; germline; homologous recombination

PMID:
23979579
PMCID:
PMC3813848
DOI:
10.1534/genetics.113.156075
[Indexed for MEDLINE]
Free PMC Article
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