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Genetics. 2013 Nov;195(3):1177-80. doi: 10.1534/genetics.113.155853. Epub 2013 Aug 26.

Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins.

Author information

1
Department of Chemistry, Seoul National University, Seoul 151-742, Korea.

Abstract

We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F1 progeny, demonstrating the high efficiency of this method.

KEYWORDS:

Caenorhabditis elegans; Cas9–sgRNA ribonucleoprotein; NHEJ; genome editing

PMID:
23979576
PMCID:
PMC3813847
DOI:
10.1534/genetics.113.155853
[Indexed for MEDLINE]
Free PMC Article

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