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Nat Rev Microbiol. 2013 Oct;11(10):728-36. doi: 10.1038/nrmicro3093. Epub 2013 Sep 2.

MLST revisited: the gene-by-gene approach to bacterial genomics.

Author information

1
Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK.

Abstract

Multilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based method for identifying clonal relationships among bacteria. Today, in the whole-genome era of microbiology, the need for systematic, standardized descriptions of bacterial genotypic variation remains a priority. Here, to meet this need, we draw on the successes of MLST and 16S rRNA gene sequencing to propose a hierarchical gene-by-gene approach that reflects functional and evolutionary relationships and catalogues bacteria 'from domain to strain'. Our gene-based typing approach using online platforms such as the Bacterial Isolate Genome Sequence Database (BIGSdb) allows the scalable organization and analysis of whole-genome sequence data.

PMID:
23979428
PMCID:
PMC3980634
DOI:
10.1038/nrmicro3093
[Indexed for MEDLINE]
Free PMC Article

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