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Nat Commun. 2013;4:2366. doi: 10.1038/ncomms3366.

In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique.

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Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.


Intravital microscopy has been used extensively to study dynamic processes in the context of a living animal; however, only a limited number of fluorescent probes and mouse models are available. By contrast, many dyes and antibodies exist for the immuno-labelling of fixed tissue. Here we report a method that combines the advantages of histochemistry and in vivo imaging by correlating cryosection labelling with corresponding intravital microscopy images (CLIM). Using CLIM, we find that the presence of CD3(+) T cells correlates with mammary tumour cell migration. When CD4(+) and CD8(+) T-cell subsets are depleted, reduced tumour cell migration is observed. From these data we conclude that CLIM is a powerful tool to correlate intravital microscopy data with cryosection labelling data.

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