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Gastroenterology. 2013 Dec;145(6):1289-99. doi: 10.1053/j.gastro.2013.08.046. Epub 2013 Aug 23.

Molecular diagnosis of eosinophilic esophagitis by gene expression profiling.

Author information

1
Division of Allergy and Immunology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio.

Abstract

BACKGROUND & AIMS:

Gene expression profiling provides an opportunity for definitive diagnosis but has not yet been well applied to inflammatory diseases. Here we describe an approach for diagnosis of an emerging form of esophagitis, eosinophilic esophagitis (EoE), which is currently diagnosed by histology and clinical symptoms.

METHODS:

We developed an EoE diagnostic panel (EDP) comprising a 96-gene quantitative polymerase chain reaction array and an associated dual-algorithm that uses cluster analysis and dimensionality reduction using a cohort of randomly selected esophageal biopsy samples from pediatric patients with EoE (n = 15) or without EoE (non-EoE controls, n = 14) and subsequently vetted the EDP using a separate cohort of 194 pediatric and adult patient samples derived from both fresh or formalin-fixed, paraffin-embedded tissue: active EoE (n = 91), control (non-EoE and EoE remission, n = 57), histologically ambiguous (n = 34), and reflux (n = 12) samples.

RESULTS:

The EDP identified adult and pediatric patients with EoE with approximately 96% sensitivity and approximately 98% specificity, and distinguished patients with EoE in remission from controls, as well as identified patients exposed to swallowed glucorticoids. The EDP could be used with formalin-fixed, paraffin-embedded tissue RNA and distinguished patients with EoE from those with reflux esophagitis, identified by pH-impedance testing. Preliminary evidence showed that the EDP could identify patients likely to have disease relapse after treatment.

CONCLUSIONS:

We developed a molecular diagnostic test (referred to as the EDP) that identifies patients with esophagitis in a fast, objective, and mechanistic manner, offering an opportunity to improve diagnosis and treatment, and a platform approach for other inflammatory diseases.

KEYWORDS:

AUC; Diagnostic Panel; EDP; EGID; EoE; EoE Transcriptome; Eosinophil; FFPE; Fluidic Card; GERD; GI; HPF; MII; NL; Nonerosive Reflux Disease; ROC; Signature; area under the curve; eosinophilic esophagitis; eosinophilic esophagitis diagnostic panel; eosinophilic gastrointestinal disorder; formalin-fixed, paraffin-embedded; gastroesophageal reflux disease; gastrointestinal; high-power field; multichannel intraluminal impedance; normal; receiver operating characteristic

PMID:
23978633
PMCID:
PMC4070519
DOI:
10.1053/j.gastro.2013.08.046
[Indexed for MEDLINE]
Free PMC Article

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