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Bioresour Technol. 2013 Oct;146:672-681. doi: 10.1016/j.biortech.2013.07.102. Epub 2013 Jul 29.

A novel production process for optically pure L-lactic acid from kitchen refuse using a bacterial consortium at high temperatures.

Author information

1
Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; Institute of Advanced Study, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
2
Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
3
Japan Eco-science Co. Ltd., 11-1 Shiomigaokacho, Chuo-ku, Chiba, Chiba 260-0034, Japan.
4
Miroku Co. Ltd., Iwaya 706-27, Kitsuki City, Oita 873-0021, Japan.
5
Laboratory of Soil Microbiology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Electronic address: kensak@agr.kyushu-u.ac.jp.

Abstract

We investigated L-lactic acid production in static batch fermentation of kitchen refuse using a bacterial consortium from marine-animal-resource (MAR) composts at temperatures ranging from 30 to 65 °C. At relatively low temperatures butyric acid accumulated, whereas at higher temperatures L-lactic acid was produced. In particular, fermentation at 50 °C produced 34.5 g L(-1) L-lactic acid with 90% lactic acid selectivity and 100% optical purity. Denaturing gradient gel electrophoresis indicated that dominant bacteria present in the original MAR composts diminished rapidly and Bacillus coagulans strains became the dominant contributors to L-lactic acid production at 45, 50 and 55 °C. This is the first report of the achievement of 100% optical purity of L-lactic acid using a bacterial consortium.

KEYWORDS:

Bacterial consortium; Kitchen refuse; l-Lactic acid fermentation

PMID:
23978480
DOI:
10.1016/j.biortech.2013.07.102
[Indexed for MEDLINE]

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