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J Nucl Med. 2013 Oct;54(10):1820-4. doi: 10.2967/jnumed.112.118497. Epub 2013 Aug 26.

Fast metabolic response to drug intervention through analysis on a miniaturized, highly integrated molecular imaging system.

Author information

  • 1NSB Cancer Center, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California.

Abstract

We report on a radiopharmaceutical imaging platform designed to capture the kinetics of cellular responses to drugs.

METHODS:

A portable in vitro molecular imaging system comprising a microchip and a β-particle imaging camera permitted routine cell-based radioassays of small numbers of either suspended or adherent cells. We investigated the kinetics of responses of model lymphoma and glioblastoma cancer cell lines to (18)F-FDG uptake after drug exposure. Those responses were correlated with kinetic changes in the cell cycle or with changes in receptor tyrosine kinase signaling.

RESULTS:

The platform enabled direct radioassays of multiple cell types and yielded results comparable to those from conventional approaches; however, the platform used smaller sample sizes, permitted a higher level of quantitation, and did not require cell lysis.

CONCLUSION:

The kinetic analysis enabled by the platform provided a rapid (≈ 1 h) drug screening assay.

KEYWORDS:

microfluidics; molecular imaging; radioassay; radiopharmaceuticals

PMID:
23978446
PMCID:
PMC4106462
DOI:
10.2967/jnumed.112.118497
[PubMed - indexed for MEDLINE]
Free PMC Article
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