Format

Send to

Choose Destination
PLoS One. 2013 Aug 15;8(8):e74511. doi: 10.1371/journal.pone.0074511. eCollection 2013.

Targeted mutagenesis in the malaria mosquito using TALE nucleases.

Author information

1
Institut National de la Santé et de la Recherche Médicale U963, Strasbourg, France.

Abstract

Anopheles gambiae, the main mosquito vector of human malaria, is a challenging organism to manipulate genetically. As a consequence, reverse genetics studies in this disease vector have been largely limited to RNA interference experiments. Here, we report the targeted disruption of the immunity gene TEP1 using transgenic expression of Transcription-Activator Like Effector Nucleases (TALENs), and the isolation of several TEP1 mutant A. gambiae lines. These mutations inhibited protein production and rendered TEP1 mutants hypersusceptible to Plasmodium berghei. The TALEN technology opens up new avenues for genetic analysis in this disease vector and may offer novel biotechnology-based approaches for malaria control.

PMID:
23977401
PMCID:
PMC3744473
DOI:
10.1371/journal.pone.0074511
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center