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Methods Mol Biol. 2013;1063:145-58. doi: 10.1007/978-1-62703-583-5_8.

Membrane protein structure determination: back to the membrane.

Author information

1
Sanford Burnham Medical Research Institute, La Jolla, CA, USA.

Abstract

NMR spectroscopy enables the structures of membrane proteins to be determined in the native-like environment of the phospholipid bilayer membrane. This chapter outlines the methods for membrane protein structural studies using solid-state NMR spectroscopy with samples of membrane proteins incorporated in proteoliposomes or planar lipid bilayers. The methods for protein expression and purification, sample preparation, and NMR experiments are described and illustrated with examples from OmpX and Ail, two bacterial outer membrane proteins that function in bacterial virulence.

PMID:
23975776
PMCID:
PMC4012560
DOI:
10.1007/978-1-62703-583-5_8
[Indexed for MEDLINE]
Free PMC Article

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