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Nat Methods. 2013 Oct;10(10):989-91. doi: 10.1038/nmeth.2610. Epub 2013 Aug 25.

DMSO enhances electrospray response, boosting sensitivity of proteomic experiments.

Author information

1
Chair of Proteomics and Bioanalytics, Center of Life and Food Sciences Weihenstephan, Technische Universität München, Freising, Germany.

Abstract

We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.

PMID:
23975139
DOI:
10.1038/nmeth.2610
[Indexed for MEDLINE]

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