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Biotechnol Lett. 2013 Nov;35(11):1831-8. doi: 10.1007/s10529-013-1291-6. Epub 2013 Aug 24.

Proteome profile of bovine ruminal epithelial tissue based on GeLC-MS/MS.

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1
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Abstract

The proteome of rumen epithelial tissue was analysed by SDS-PAGE coupled with LC-MS/MS. 813 non-redundant proteins were identified of which 7.4 % featured membrane-spanning domains and 15.4 % harboured a signal peptide. According to the gene ontology annotation, the most abundant proteins exhibited binding activities related to their molecular functions, were proteins of cellular components or belonged to various metabolic processes. A predominant group of canonical pathways in the rumen epithelial tissue was identified using the IPA software. The GeLC-MS/MS approach was used to characterise the entire protein expression repertoire in rumen tissue, providing a more detailed understanding of the important biological processes in the rumen.

PMID:
23974490
DOI:
10.1007/s10529-013-1291-6
[Indexed for MEDLINE]
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