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Insect Biochem Mol Biol. 2013 Nov;43(11):1009-14. doi: 10.1016/j.ibmb.2013.08.003. Epub 2013 Aug 22.

Regulation of atrophin by both strands of the mir-8 precursor.

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Institute of Evolutionary Biology (CSIC - Universitat Pompeu Fabra), Passeig Maritim de la Barceloneta 37, 0803 Barcelona, Spain.


In Drosophila melanogaster, miR-8-3p regulates mRNA levels of atrophin, a factor involved in neuromotor coordination, and we found that Blattella germanica with suppressed atrophin showed motor problems. Bionformatic predictions and luciferase-reporter tests indicated that B. germanica atrophin mRNA contains target sites for miR-8-3p and miR-8-5p. Suppression of miR-8-3p or miR-8-5p appeared to increase atrophin mRNA. The effects of suppression of Argonaute (AGO) 1 or AGO2 expression on miR-8-3p and miR-8-5p suggested that miR-8-3-p might predominantly bind to AGO1, whereas miR-8-5p might bind to a moderate extent to both AGO1 and AGO2 in the respective RNA-induced silencing complexes (RISCs). We propose that the interplay of miR-8-3p, miR-8-5p, AGO1 and AGO2, maintain the appropriate levels of atrophin mRNA. This would be the first example of two strands of the same miRNA precursor regulating a single transcript.


Argonaute; Atrophin; Blattella; Drosophila; Metamorphosis; miR-8; miRNA passenger strand

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