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Anal Biochem. 2013 Dec 1;443(1):16-21. doi: 10.1016/j.ab.2013.08.011. Epub 2013 Aug 21.

A chemiluminescence method to detect malondialdehyde in plasma and urine.

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School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061 Shaanxi, People's Republic of China; Institute of Analytical Science, Xi'an Jiaotong University, Xi'an, 710061 Shaanxi, People's Republic of China.


A highly sensitive and convenient method to detect malondialdehyde (MDA) in specimens of plasma and urine was developed using high-performance liquid chromatography combining chemiluminescence detection. MDA was separated through a reverse-phase C18 column at a flow rate of 0.8 ml min(-1). It was then detected by a chemiluminescence detector. Variables that affected chemiluminescence reaction, including mobile phase, flow rate, chemiluminescence reagent, parameters of the photomultiplier tube, and temperature, were studied. This assay was linear from 0.50 to 50 μmol L(-1), with limits of detection and quantification of 0.08 and 0.30 μmol L(-1), respectively. The recoveries were in the range of 92.2 to 98.5%, and the intra- and interday reproducibilities were obtained with relative standard deviations of less than 4% and less than 6%, respectively. MDA in plasma and urine was stable for 12 h at 4°C. MDA levels were 2.74, 3.19, and 3.20 μmol L(-1) for plasma and 1.25, 2.04, and 1.58 μmol L(-1) for urine, respectively. The proposed method is simple, rapid, and sensitive with low cost. Taken together, this method can be suitable for detecting MDA in tissue and feed specimens.


High-performance liquid chromatography–chemiluminescence; Malondialdehyde; Plasma; Urine

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