Format

Send to

Choose Destination
J Lab Autom. 2013 Dec;18(6):519-29. doi: 10.1177/2211068213499917. Epub 2013 Aug 22.

Development of a multiplexed microfluidic platform for the automated cultivation of embryonic stem cells.

Author information

1
1Department of Biochemical Engineering, University College London, London, UK.

Abstract

We present a multiplexed platform for a microfabricated stem cell culture device. The modular platform contains all the components to control stem cell culture conditions in an automated fashion. It does not require an incubator during perfusion culture and can be mounted on the stage of an inverted fluorescence microscope for high-frequency imaging of stem cell cultures. A pressure-driven pump provides control over the medium flow rate and offers switching of the flow rates. Flow rates of the pump are characterized for different pressure settings, and a linear correlation between the applied pressure and the flow rate in the cell culture devices is shown. In addition, the pump operates with two culture medium reservoirs, thus enabling the switching of the culture medium on-the-fly during a cell culture experiment. Also, with our platform, the culture medium reservoirs are cooled to prevent medium degradation during long-term experiments. Media temperature is then adjusted to a higher controlled temperature before entering the microfabricated cell culture device. Furthermore, the temperature is regulated in the microfabricated culture devices themselves. Preliminary culture experiments are demonstrated using mouse embryonic stem cells.

KEYWORDS:

bioprocess microfluidics; microfabricated culture device; multiplexing; stem cell culture

PMID:
23970473
PMCID:
PMC4107755
DOI:
10.1177/2211068213499917
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center