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Chem Soc Rev. 2014 Feb 21;43(4):1007-13. doi: 10.1039/c3cs60184f.

Maximizing information content of single-molecule FRET experiments: multi-color FRET and FRET combined with force or torque.

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1
Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Korea. shohng@snu.ac.kr.

Abstract

Since its first demonstration about twenty years ago, single-molecule fluorescence resonance energy transfer (FRET) has undergone remarkable technical advances. In this tutorial review, we will discuss two technical advances that increase the information content of the single-molecule FRET measurements: single-molecule multi-color FRET and single-molecule FRET combined with force or torque. Our expectations for future developments will be briefly discussed at the end.

PMID:
23970315
DOI:
10.1039/c3cs60184f
[Indexed for MEDLINE]
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