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FEBS Lett. 2013 Oct 1;587(19):3195-201. doi: 10.1016/j.febslet.2013.07.056. Epub 2013 Aug 19.

Attenuation of fibroblast growth factor signaling by poly-N-acetyllactosamine type glycans.

Author information

1
Department of Gynecology and Obstetrics, Hamamatsu University School of Medicine, Hamamatsu City, Shizuoka, Japan. Electronic address: sugihara@hama-med.ac.jp.

Abstract

Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glycans (polyLacs), enhanced motility. Mass spectrometry analysis revealed that sperm-associated polyLacs are heavily fucosylated, consistent with Lewis Y antigen. Immunohistochemistry of epididymis using an anti-Lewis Y antibody before and after EBG treatment suggested that polyLacs carrying the Lewis Y epitope are synthesized in epididymal epithelia and secreted to seminal fluid. EBG-treated sperm elevated cAMP levels and calcium influx, indicating activation of fibroblast growth factor signaling. Seminal fluid polyLacs bound to FGFs in vitro, and impaired FGF-mediated signaling in HEK293T cells.

KEYWORDS:

Glycan array; Heparin; Keratan sulfate; MALDI-MS; Tyrosine phosphorylation

PMID:
23968720
PMCID:
PMC4029356
DOI:
10.1016/j.febslet.2013.07.056
[Indexed for MEDLINE]
Free PMC Article

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