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Cell Cycle. 2013 Sep 1;12(17):2774-80. doi: 10.4161/cc.25872. Epub 2013 Aug 6.

Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells.

Author information

1
AntiCancer, Inc; San Diego, CA USA; Department of Surgery; University of California San Diego; San Diego, CA USA; Yokohama City University Graduate School of Medicine; Yokohama, Japan.

Abstract

The XPA1 human pancreatic cancer cell line is dimorphic, with spindle stem-like cells and round non-stem cells. We report here the in vitro IC 50 values of stem-like and non-stem XPA1 human pancreatic cells cells for: (1) 5-fluorouracil (5-FU), (2) cisplatinum (CDDP), (3) gemcitabine (GEM), and (4) tumor-targeting Salmonella typhimurium A1-R (A1-R). IC 50 values of stem-like XPA1 cells were significantly higher than those of non-stem XPA1 cells for 5-FU (P = 0.007) and CDDP (P = 0.012). In contrast, there was no difference between the efficacy of A1-R on stem-like and non-stem XPA1 cells. In vivo, 5-FU and A1-R significantly reduced the tumor weight of non-stem XPA1 cells (5-FU; P = 0.028; A1-R; P = 0.011). In contrast, only A1-R significantly reduced tumor weight of stem-like XPA1 cells (P = 0.012). The combination A1-R with 5-FU improved the antitumor efficacy compared with 5-FU monotherapy on the stem-like cells (P = 0.004). The results of the present report indicate A1-R is a promising therapy for chemo-resistant pancreatic cancer stem-like cells.

KEYWORDS:

GFP; RFP; Salmonella typhimurium; amino acid auxotrophy; chemoresistance; confocal microscopy; fluorescence imaging; pancreatic cancer; selective tumor targeting; stem cell

PMID:
23966167
PMCID:
PMC3899191
DOI:
10.4161/cc.25872
[Indexed for MEDLINE]
Free PMC Article

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