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Methods Mol Biol. 2013;1061:59-77. doi: 10.1007/978-1-62703-589-7_3.

Antigen identification using SEREX.

Author information

1
Translational Oncology (TRON), Mainz, Germany.

Abstract

Serological analysis of recombinant cDNA expression libraries (SEREX) allows systematic identification of antigens recognized by the spontaneous autoantibody repertoire of patients with cancer and autoimmune disease. SEREX is based on screening of lambda phage expression libraries constructed from diseased tissue with the autologous patient's serum. This chapter provides the key protocols for SEREX immunoscreening and for downstream validation of autoantigens.

PMID:
23963930
DOI:
10.1007/978-1-62703-589-7_3
[Indexed for MEDLINE]

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