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Cancer Lett. 2013 Dec 1;341(2):240-7. doi: 10.1016/j.canlet.2013.08.021. Epub 2013 Aug 17.

MiR-101 inhibits melanoma cell invasion and proliferation by targeting MITF and EZH2.

Author information

1
Department of Translational Immunology, German Cancer Research Center (DKFZ), Heidelberg, Germany. Electronic address: c.luo@dkfz.de.

Abstract

The microRNA miR-101 has been reported to be a tumor suppressor. Here we show that low expression of miR-101 is associated with poor survival in stage IV melanoma patients. We identified microphthalmia-associated transcription factor (MITF) as a direct target of miR-101. In melanoma cells, overexpression of miR-101 downregulated protein levels of MITF and a previously reported target protein, enhancer of zeste homolog 2 (EZH2). Functional assays showed that miR-101 suppressed invasion and proliferation - an outcome that could be phenocopied by siRNA knockdown of MITF and EZH2. Our data suggest that miR-101 might have a beneficial role in melanoma.

KEYWORDS:

Enhancer of zeste homolog 2; Invasion; Melanoma; MicroRNA; Microphthalmia-associated transcription factor

PMID:
23962556
DOI:
10.1016/j.canlet.2013.08.021
[Indexed for MEDLINE]

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